6STE
Crystal structure of the tick chemokine-binding protein Evasin-4 (SG 3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97717 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.214, 68.214, 181.531 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.175 - 1.790 |
R-factor | 0.1808 |
Rwork | 0.179 |
R-free | 0.20960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6st4 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.070 | 1.850 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.200 | 0.101 |
Number of reflections | 47147 | 3398 |
<I/σ(I)> | 7.1 | 3 |
Completeness [%] | 100.0 | |
Redundancy | 19.4 | |
CC(1/2) | 0.988 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 15% glycerol, 25.5% PEG 4000, 0.17 M ammonium acetate, 85 mM acetate buffer pH 4.6 |