6STA

Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein, E46A D48A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-23
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	58.138, 75.433, 83.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.860 - 2.190
R-factor	0.215
Rwork	0.213
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5amw
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.856	2.396
High resolution limit [Å]	2.195	2.195
Rmerge	0.138	1.466
Rmeas	0.149
Rpim	0.054	0.563
Number of reflections	14982	749
<I/σ(I)>	11	1.4
Completeness [%]	92.2	45.1
Redundancy	7.3	7.7
CC(1/2)	0.998	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M sodium citrate tribasic