6ST8
Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.072274 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.920, 41.730, 91.350 |
| Unit cell angles | 90.00, 117.14, 90.00 |
Refinement procedure
| Resolution | 47.130 - 2.040 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5amw |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.450 | 2.270 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.071 | 0.783 |
| Rmeas | 0.085 | 0.957 |
| Rpim | 0.047 | 0.541 |
| Number of reflections | 15170 | 760 |
| <I/σ(I)> | 8.7 | 1.1 |
| Completeness [%] | 88.1 | 49.6 |
| Redundancy | 3.2 | 2.7 |
| CC(1/2) | 0.998 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Bis-Tris pH5.5 140mM Ammonium Sulfate 33% PEG3350 |
