6SSY
Taurine ABC transporter substrate binding protein TauA from E. coli in complex with 2-Aminoethylphosphonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.100, 71.640, 164.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.680 - 1.620 |
| Rwork | 0.168 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6stl |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.680 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.100 | 0.860 |
| Number of reflections | 68655 | 3375 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.3 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M sodium iodide, 0.1 M Bis-Tris Propane, 20% (w/v) PEG 3350 + 10 mM 2-AEP |
