6SSX
cryo-em structure of alpha-synuclein fibril polymorph 2A
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.98 |
| Software | RELION,PHENIX,Coot,SerialEM,FOCUS,MotionCorr2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
