6SSO

EDN mutant L45H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979256
Spacegroup name	P 21 21 21
Unit cell lengths	42.120, 52.470, 56.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.489 - 1.211
R-factor	0.1355
Rwork	0.135
R-free	0.15120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gqv
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.490	1.270
High resolution limit [Å]	1.210	1.210
Number of reflections	38717	1897
<I/σ(I)>	6.6
Completeness [%]	99.6
Redundancy	4.1
CC(1/2)	0.976	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	297	100 mM sodium cacodylate, pH 7 and 1.6 M sodium acetate