6SSN
RNASE 3/1 version3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979280 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.381, 39.548, 46.331 |
| Unit cell angles | 73.61, 83.11, 66.64 |
Refinement procedure
| Resolution | 35.235 - 1.511 |
| R-factor | 0.1759 |
| Rwork | 0.173 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.235 | 1.560 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 31951 | 3156 |
| <I/σ(I)> | 7.98 | 1.59 |
| Completeness [%] | 84.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.994 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium citrate, pH 6.5 and 1.5 M ammonium phosphate |
