6SRV
Structure of the arginase-2-inhibitory human antigen-binding fragment Fab C0021144
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979500 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 172.690, 172.690, 211.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.901 - 2.400 |
| Rwork | 0.205 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.769 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.901 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.360 | 9.900 |
| Rmeas | 10.190 | |
| Rpim | 0.066 | 2.226 |
| Number of reflections | 47369 | 4600 |
| <I/σ(I)> | 7.6 | 0.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 29.5 | 20.9 |
| CC(1/2) | 1.000 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200 mM NH4Cl 20% PEG3350 |
