6SRE
Crystal Structure of Human Prolidase S202F variant expressed in the presence of chaperones
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.725, 106.858, 216.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.765 - 1.390 |
| R-factor | 0.1501 |
| Rwork | 0.150 |
| R-free | 0.17230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m4j |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.765 | 1.470 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmeas | 0.097 | 1.756 |
| Number of reflections | 239232 | 38067 |
| <I/σ(I)> | 10.32 | 0.74 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 7.16 | 5.5 |
| CC(1/2) | 0.998 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 10mM NaBorate, 760mM NaCitrate |
