6SR6

Crystal structure of the RAC core with a pseudo substrate bound to Ssz1 SBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	51.977, 258.454, 53.017
Unit cell angles	90.00, 100.09, 90.00

Refinement procedure

Resolution	33.710 - 2.500
Rwork	0.189
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mb9
Data reduction software	XDS (20180126)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.400	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.134	0.706
Number of reflections	46860	4516
<I/σ(I)>	9.6	2.2
Completeness [%]	98.9
Redundancy	6
CC(1/2)		0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20.5% (v/v) PEG 3350, 0.2 M ammonium acetate