6SR2
X-ray pump X-ray probe on lysozyme.Gd nanocrystals: 37 fs time delay
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.75 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.000, 79.000, 39.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.810 - 2.300 |
| R-factor | 0.2352 |
| Rwork | 0.229 |
| R-free | 0.29430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n5r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.512 |
| Data reduction software | CrystFEL |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.810 | 2.360 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 5315 | 436 |
| <I/σ(I)> | 12.4 | 11.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 1 | |
| CC(1/2) | 0.981 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 3 | 277 | 20 % NACL, 6 % PEG 6000, 0.1 M SODIUM acetate pH 3.0 |
