6SQW
Mouse dCTPase in complex with 5-Me-dCMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.07 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.516, 58.516, 140.905 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.040 - 1.800 |
R-factor | 0.193 |
Rwork | 0.192 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.596 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.500 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.130 | 1.360 |
Number of reflections | 23533 | 32921 |
<I/σ(I)> | 12.2 | 1.7 |
Completeness [%] | 100.0 | |
Redundancy | 13.9 | |
CC(1/2) | 0.990 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 8.75, 2.4 M ammonium sulfate |