6SQH

Crystal structure of mouse PRMT6 with partial C-terminal TEV cleavage site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-05
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	41.143, 118.106, 71.760
Unit cell angles	90.00, 103.24, 90.00

Refinement procedure

Resolution	36.851 - 2.390
R-factor	0.2116
Rwork	0.209
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c03
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.102	0.691
Rmeas	0.117	0.800
Rpim	0.056	0.396
Number of reflections	26129	2472
<I/σ(I)>	13.1	2
Completeness [%]	98.8	89.2
Redundancy	4.1	3.7
CC(1/2)	0.996	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	PEG Smear Broad 22%, MES pH 6.5 100 mM