6SP0

Structure of Esco2 acetyltransferase in complex with CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-30
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	52.680, 52.680, 107.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.620 - 1.770
Rwork	0.169
R-free	0.19800
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	1.810
High resolution limit [Å]	1.770	1.770
Number of reflections	28388	1533
<I/σ(I)>	15.2	4.7
Completeness [%]	99.7	95.8
Redundancy	6.7	6.5
CC(1/2)	0.990	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	100 mM Tris, 20% (v/v) and 2-Methyl-2,4-pentanediol (MPD)