6SOA
BamABCDE in MSP1D1 nanodisc ensemble 0-9
EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.6 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 10.8 |
Software | RELION,PHENIX,EPU,Gctf,UCSF Chimera,MDFF |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |