6SNQ
Crystal structures of human PGM1 isoform 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.262, 53.155, 76.825 |
Unit cell angles | 90.00, 99.14, 90.00 |
Refinement procedure
Resolution | 48.630 - 2.700 |
R-factor | 0.2079 |
Rwork | 0.206 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.481 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MrBUMP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.630 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.082 | 0.560 |
Rmeas | 0.092 | 0.632 |
Rpim | 0.043 | 0.286 |
Number of reflections | 16247 | 2160 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 99.6 | |
Redundancy | 4.6 | |
CC(1/2) | 0.997 | 0.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | KCl, MgCl2, Na cacodylate pH 6.5 and PEG4000 |