6SNK
Crystal structure of the Collagen VI alpha3 N2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 |
Unit cell lengths | 36.639, 40.538, 57.114 |
Unit cell angles | 83.34, 76.79, 79.56 |
Refinement procedure
Resolution | 55.430 - 2.200 |
R-factor | 0.2031 |
Rwork | 0.200 |
R-free | 0.25750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4igi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.648 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3594) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.452 | 55.430 | 2.270 |
High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
Rmerge | 0.221 | 0.146 | 1.240 |
Rmeas | 0.267 | 0.174 | 1.546 |
Rpim | 0.147 | 0.094 | 0.903 |
Number of reflections | 15370 | 225 | 1341 |
<I/σ(I)> | 0.939 | ||
Completeness [%] | 96.5 | 99.1 | 96.5 |
Redundancy | 3.4 | 3.3 | 3.2 |
CC(1/2) | 0.951 | 0.225 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 298 | 30% P550MME_P20K, 0.1M Morpheus buffer 1, 0.12M Morpheus alcohols (condition D1 of Morpheus screen from Molecular Dimensions |