6SMV
Structure of HPV49 E6 protein in complex with MAML1 LxxLL motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-17 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.906, 44.539, 94.237 |
Unit cell angles | 90.00, 99.70, 90.00 |
Refinement procedure
Resolution | 46.440 - 2.140 |
R-factor | 0.2197 |
Rwork | 0.218 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4giz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.435 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18rc1_3769) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.440 | 2.220 |
High resolution limit [Å] | 2.140 | 2.144 |
Number of reflections | 63547 | 10215 |
<I/σ(I)> | 11.14 | |
Completeness [%] | 97.7 | |
Redundancy | 7.6 | |
CC(1/2) | 0.998 | 0.688 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.8 | 277 | Lithium Acetate 200mM, PEG 3350 22.5% |