6SMK
Crystal structure of catalytic domain A109H mutant of prophage-encoded M23 protein EnpA from Enterococcus faecalis.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.911600 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 136.469, 136.469, 325.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.246 - 2.997 |
R-factor | 0.2263 |
Rwork | 0.225 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3slu |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.246 | 49.246 | 3.180 |
High resolution limit [Å] | 2.997 | 8.860 | 3.000 |
Rmerge | 0.211 | 0.039 | 2.930 |
Rmeas | 0.216 | 0.041 | 2.997 |
Number of reflections | 36807 | 1622 | 5779 |
<I/σ(I)> | 15.58 | 63.43 | 1.05 |
Completeness [%] | 99.8 | 99 | 99.2 |
Redundancy | 21.806 | 17.894 | 22.548 |
CC(1/2) | 0.999 | 0.999 | 0.434 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 0.8M succinic acid, pH 7.0 |