6SLL
Diaminobutyrate acetyltransferase EctA from Paenibacillus lautus in complex with its substrate L-2,4-diaminobutyric acid (DAB) and coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | H 3 |
| Unit cell lengths | 151.048, 151.048, 46.172 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.520 - 1.200 |
| R-factor | 0.1232 |
| Rwork | 0.122 |
| R-free | 0.14960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sk1 |
| RMSD bond length | 0.039 |
| RMSD bond angle | 3.024 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.530 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 182408 | 27168 |
| <I/σ(I)> | 10.43 | 1.59 |
| Completeness [%] | 99.2 | |
| Redundancy | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.2 M sodium fluoride, 20 % (w/v) PEG 3350, 5 mM coenzyme A, 20 mM L-2,4-diaminobutyrate |
