6SLC
Mutations in SsgB correlate to longitudinal cell division during sporulation of Streptomyces coelicolor
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.975 |
Spacegroup name | I 4 |
Unit cell lengths | 155.471, 155.471, 53.753 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.213 - 2.300 |
Rwork | 0.208 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cm1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.413 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.927 | 2.309 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.065 | 1.009 |
Rmeas | 0.076 | 1.167 |
Rpim | 0.037 | 0.570 |
Number of reflections | 33694 | 1579 |
<I/σ(I)> | 9.4 | 1.1 |
Completeness [%] | 95.5 | 92.1 |
Redundancy | 4 | 4.1 |
CC(1/2) | 0.979 | 0.477 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Sodium chloride, 0.1 M Sodium/potassium phosphate (pH 6.2), 50% PEG200 |