6SL7
The Delta Calcium mutant of ALPHA-ACTININ FROM ENTAMOEBA HISTOLYTICA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.968620 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 40.739, 75.526, 234.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.320 - 3.300 |
| R-factor | 0.23711 |
| Rwork | 0.234 |
| R-free | 0.30810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sl2 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.850 | 3.380 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmeas | 0.084 | |
| Number of reflections | 11494 | 2235 |
| <I/σ(I)> | 11.57 | |
| Completeness [%] | 97.8 | 96.2 |
| Redundancy | 4.6 | 5.8 |
| CC(1/2) | 0.998 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M bis-tris (pH 5.5), 0.3 M MgCl2, 20% (w/v) PEG 3,350 |
