6SL7

The Delta Calcium mutant of ALPHA-ACTININ FROM ENTAMOEBA HISTOLYTICA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.968620
Spacegroup name	P 2 2 21
Unit cell lengths	40.739, 75.526, 234.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.320 - 3.300
R-factor	0.23711
Rwork	0.234
R-free	0.30810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sl2
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.850	3.380
High resolution limit [Å]	3.300	3.300
Rmeas	0.084
Number of reflections	11494	2235
<I/σ(I)>	11.57
Completeness [%]	97.8	96.2
Redundancy	4.6	5.8
CC(1/2)	0.998	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M bis-tris (pH 5.5), 0.3 M MgCl2, 20% (w/v) PEG 3,350