6SL4
The unique CBM-Cthe_0271 of Ruminiclostridium thermocellum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-07 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.990, 77.990, 288.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.540 - 1.800 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.541 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 46318 | 2494 |
| <I/σ(I)> | 17.7 | 1.3 |
| Completeness [%] | 99.0 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.999 | 0.398 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M Hepes buffer ph7.5, 4.3M sodium cloride |
