6SL2

ALPHA-ACTININ FROM ENTAMOEBA HISTOLYTICA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97860
Spacegroup name	P 42 21 2
Unit cell lengths	192.262, 192.262, 57.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.640 - 3.100
Rwork	0.215
R-free	0.25140
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.640	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.109
Number of reflections	20112	3580
<I/σ(I)>	13.6	1.3
Completeness [%]	99.2	100
Redundancy	8.7
CC(1/2)	0.984	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	92 mM bicine/trizma base (pH 8.5), 27.6 mM CaCl2, 27.6 mM MgCl2, 11.5% (w/v) PEG 3,350, 11.5% (w/v) PEG 1,000, 11.5% (v/v) MPD