6SKE
Teneurin 2 in complex with Latrophilin 2 Lec domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979560 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.870, 109.920, 152.210 |
| Unit cell angles | 90.19, 93.99, 111.93 |
Refinement procedure
| Resolution | 84.000 - 3.620 |
| R-factor | 0.27 |
| Rwork | 0.270 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.000 | 3.710 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 59561 | 4393 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 96.0 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.970 | 0.250 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.5, 10% (w/v) PEG 4000 |
