6SJT
Crystal structure of the Legionella pneumophila type II secretion system substrate NttC
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9796, 0.9797, 0.9681 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 84.390, 84.390, 151.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.084 - 3.103 |
| Rwork | 0.250 |
| R-free | 0.28860 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.084 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.283 | 1.022 |
| Rmeas | 0.290 | 1.048 |
| Rpim | 0.064 | 0.226 |
| Number of reflections | 6280 | 437 |
| <I/σ(I)> | 10.1 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.4 | 20.9 |
| CC(1/2) | 0.994 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 3.5 M ammonium chloride, 100 mM Tris-HCl pH 8.0 |
