6SJH

Structure of the PRY-SPRY domain of human Trim16L/Trim70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	72.780, 95.770, 116.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.1821
Rwork	0.181
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b8e
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.600
High resolution limit [Å]	1.500	1.500
Rmerge	0.075
Rmeas		0.555
Number of reflections	221803	95480
<I/σ(I)>	7.04
Completeness [%]	88.3	92.1
Redundancy	2.48	2.34
CC(1/2)	0.993	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	Na-acetate, PEG 400, PGA