6SJ9

Proteasome accessory factor B/C (PafBC) of Arthrobacter aurescens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978561
Spacegroup name	P 21 21 21
Unit cell lengths	77.875, 119.033, 160.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.725 - 2.200
R-factor	0.2049
Rwork	0.203
R-free	0.23750
Structure solution method	SAD
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180808)
Data scaling software	Aimless (0.7.3)
Phasing software	SHELXCD (2016/1)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.725	48.725	2.140
High resolution limit [Å]	2.100	11.320	2.100
Rmerge	0.144	0.056	5.340
Rmeas	0.145	0.057	5.385
Rpim	0.017	0.007	0.684
Number of reflections	87298	655	4299
<I/σ(I)>	27.3
Completeness [%]	99.9	98.9	97.5
Redundancy	67.7	61.1	58.9
CC(1/2)	1.000	1.000	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	100 mM Bis-Tris-propane pH 8.8 to 9.2, 80-160 mM KSCN, 18-21% w/v PEG-2000