6SIR
Crystal structure of the guanylate cyclase domain of RhGC from Catenaria anguillulae in complex with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.650, 95.170, 138.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.950 - 1.700 |
R-factor | 0.1831 |
Rwork | 0.182 |
R-free | 0.20940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w01 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.816 |
Data reduction software | DIALS (1.10) |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.520 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.073 | 1.270 |
Rmeas | 0.076 | 1.320 |
Rpim | 0.021 | 0.360 |
Number of reflections | 85155 | 4426 |
<I/σ(I)> | 20.3 | 2.2 |
Completeness [%] | 99.0 | 98 |
Redundancy | 13.2 | 13.5 |
CC(1/2) | 1.000 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MIB pH 4.4, 22% PEG 2000 |