6SIM
SAD structure of Hen Egg White Lysozyme recovered by inverse beam geometry data collection and univariate analysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.54980 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.070, 79.070, 36.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.535 - 1.610 |
R-factor | 0.147399816682 |
Rwork | 0.146 |
R-free | 0.17377 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.779 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.535 | 1.650 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.064 | 0.277 |
Number of reflections | 28213 | 1392 |
<I/σ(I)> | 44.83 | 3.19 |
Completeness [%] | 97.3 | 65.7 |
Redundancy | 38.1 | 3.4 |
CC(1/2) | 1.000 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293.15 | 50mM Na Acetate pH 4.5 1M NaCl 25% Ethylene glycol |