6SIK
SAD structure of Hen Egg White Lysozyme recovered by continuous rotation data collection and univariate analysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.54980 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.870, 78.870, 36.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.435 - 1.610 |
| R-factor | 0.140491952434 |
| Rwork | 0.139 |
| R-free | 0.16270 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.435 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.079 | 0.223 |
| Number of reflections | 28771 | 2102 |
| <I/σ(I)> | 47.53 | 8.84 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 39.2 | 5.8 |
| CC(1/2) | 1.000 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293.15 | 50mM Na acetate pH 4.5 1M NaCl 25% Ethylene glycol |
