6SIJ

SAD structure of Hen Egg White Lysozyme recovered by continuous rotation data collection and multivariate analysis of Friedel pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.54980
Spacegroup name	P 43 21 2
Unit cell lengths	79.070, 79.070, 36.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.535 - 1.610
R-factor	0.141874113995
Rwork	0.141
R-free	0.16733
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.535	1.650
High resolution limit [Å]	1.610	1.610
Rmerge	0.079	0.223
Number of reflections	28771	2102
<I/σ(I)>	47.53	8.84
Completeness [%]	99.9	99.2
Redundancy	39.2	5.8
CC(1/2)	1.000	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293.15	50mM Na acetate pH 4.5 1M NaCl 25% Ethylene glycol