6SIF
Epidermicin antimicrobial protein from Staphylococcus epidermidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0725 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.180, 117.860, 52.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.130 - 1.690 |
| R-factor | 0.2482 |
| Rwork | 0.247 |
| R-free | 0.26200 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.130 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.058 | 0.576 |
| Rmeas | 0.075 | 0.744 |
| Rpim | 0.035 | 0.351 |
| Number of reflections | 64599 | 4639 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.0 | 97.6 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium Sulphate, 0.1M Sodium Acetate pH4.5, 28% PEG 2000 MME |
