6SI9
FtsZ-refold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.731, 50.057, 88.694 |
| Unit cell angles | 90.00, 111.71, 90.00 |
Refinement procedure
| Resolution | 41.200 - 1.900 |
| R-factor | 0.1762 |
| Rwork | 0.173 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rvm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.417 |
| Rmeas | 0.078 | 0.590 |
| Rpim | 0.005 | 0.417 |
| Number of reflections | 22836 | 2265 |
| <I/σ(I)> | 7.52 | 1.45 |
| Completeness [%] | 96.8 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.995 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | Lithium sulfate, ethyleneglycol, Tris-HCl, PEG-MME 5000 |
