6SI9
FtsZ-refold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 72.731, 50.057, 88.694 |
Unit cell angles | 90.00, 111.71, 90.00 |
Refinement procedure
Resolution | 41.200 - 1.900 |
R-factor | 0.1762 |
Rwork | 0.173 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvm |
RMSD bond length | 0.006 |
RMSD bond angle | 0.879 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.200 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.055 | 0.417 |
Rmeas | 0.078 | 0.590 |
Rpim | 0.005 | 0.417 |
Number of reflections | 22836 | 2265 |
<I/σ(I)> | 7.52 | 1.45 |
Completeness [%] | 96.8 | |
Redundancy | 1.9 | |
CC(1/2) | 0.995 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | Lithium sulfate, ethyleneglycol, Tris-HCl, PEG-MME 5000 |