6SI6

N-terminal domain of Drosophila X virus VP3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97166
Spacegroup name	P 41 21 2
Unit cell lengths	45.334, 45.334, 104.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.591 - 1.980
R-factor	0.1756
Rwork	0.174
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6shw
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (dev_3084)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	2.051
High resolution limit [Å]	1.980	1.980
Rmerge	0.055
Rpim	0.020
Number of reflections	8128	780
<I/σ(I)>	23.01
Completeness [%]	99.9
Redundancy	7.7
CC(1/2)	0.999	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG 4K, 200 mM ammonium sulphate and 100 mM sodium acetate pH 4.6