6SHZ
p53 cancer mutant Y220C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97624 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.182, 71.154, 105.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.487 - 1.240 |
R-factor | 0.1523 |
Rwork | 0.151 |
R-free | 0.17250 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2j1x |
RMSD bond length | 0.005 |
RMSD bond angle | 0.805 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.973 | 1.310 |
High resolution limit [Å] | 1.240 | 1.240 |
Rmerge | 0.053 | 0.582 |
Number of reflections | 135992 | 19284 |
<I/σ(I)> | 15.6 | 3.1 |
Completeness [%] | 98.0 | 96.2 |
Redundancy | 5.5 | 5.5 |
CC(1/2) | 0.999 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein solution: 6 mg/ml protein in 25 mM sodium phosphate, ph 7.2, 150 mm KCl, 5 mm DTT. Reservoir buffer: 100 mm HEPES, pH 7.2, 19% (w/v) polyethylene glycol 4000, 5 mm DTT. |