6SHW

N-terminal domain of Drosophila X Virus VP3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 63 2 2
Unit cell lengths	77.331, 77.331, 48.054
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.043 - 2.000
R-factor	0.1697
Rwork	0.167
R-free	0.22060
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	Arcimboldo
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.050	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.070
Number of reflections	6029	526
<I/σ(I)>	34.49
Completeness [%]	98.5	88.51
Redundancy	19.4
CC(1/2)	1.000	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG 4K, 200 mM ammonium sulphate and 100 mM sodium acetate pH 4.6