6SFW
Cryo-EM Structure of the ClpX component of the ClpXP1/2 degradation machinery.
EM specimen
Vitrification | Yes |
Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 6 |
Number of Particle | 613322 |
Software | SPHIRE,NAMD,Rosetta,PHENIX,Coot,EPU,UCSF Chimera |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |