6SFT

Solution structure of protein ARR_CleD in complex with c-di-GMP

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	DRX		600
2	Bruker	DRX		900
3	Bruker	DRX		800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	2	3D C(CO)NH
11	1	2	3D CBCANH
12	1	2	3D HBHA(CO)NH
13	1	2	3D CBCA(CO)NH
14	1	1	3D 15N t2 interleaved
15	1	2	2D 1H-1H NOESY isotpe filtered
16	1	1	3D 15N t1 interleaved
17	1	1	2D 1H-15N NOE without sturation
18	2	2	3D HN(CO)CA without decoupling
19	1	1	2D 1H-15N NOE with sturation
2	1	1	2D 1H-15N HSQC
23	2	2	2D 1H-15N HSQC IPAP
24	1	2	3D HN(CO)CA
25	1	2	2D 1H-15N HSQC IPAP
3	1	1	3D 1H-15N NOESY
4	1	1	3D 1H-15N TOCSY
5	1	1	3D 1H-15N NH2 NOESY
6	1	1	2D 1H-1H NOESY
7	1	2	3D HNCO
8	1	2	3D HNCA
9	1	1	3D 1H-15N NH2 TOCSY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	1	298
2	6.5	1	298

Conformers

Conformers Calculated Total Number	200
Conformers Submitted Total Number	10