6SF7
Atomic resolution structure of SplF protease from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-05-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.000, 58.580, 62.300 |
| Unit cell angles | 104.57, 95.31, 90.60 |
Refinement procedure
| Resolution | 29.840 - 1.700 |
| R-factor | 0.1694 |
| Rwork | 0.167 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4VID |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.050 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.047 | 0.110 |
| Number of reflections | 77687 | 10882 |
| <I/σ(I)> | 6.4 | 13.9 |
| Completeness [%] | 92.6 | 88.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 32.5 % PEG 400, 0.3 M Ammonium Sulphate pH = 3.5 |
