6SEO
TEAD4 bound to a FAM181B peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 135.850, 135.850, 88.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.920 - 2.550 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ge3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.507 | 2.643 |
| High resolution limit [Å] | 2.521 | 2.521 |
| Rmerge | 0.112 | 2.249 |
| Number of reflections | 7784 | 389 |
| <I/σ(I)> | 15.5 | 1.1 |
| Completeness [%] | 72.5 | 28 |
| Redundancy | 10.1 | 10.2 |
| CC(1/2) | 0.999 | 0.421 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50 mM ammonium sulfate 50 mM Bis-TRIS pH 6.5 30% Pentaerythritol ethoxylate (15/4 EO/OH) |
