6SEI
Recognition and processing of branched DNA substrates by Slx1-Slx4 nuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.075, 92.700, 93.251 |
Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
Resolution | 46.610 - 2.690 |
R-factor | 0.2152 |
Rwork | 0.208 |
R-free | 0.26550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6seh |
RMSD bond length | 0.004 |
RMSD bond angle | 0.637 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.610 | 2.850 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 28286 | 4554 |
<I/σ(I)> | 7.23 | 2.44 |
Completeness [%] | 98.9 | 98.8 |
Redundancy | 3.49 | 3.59 |
CC(1/2) | 0.985 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298.15 | 0.1 M MES (pH 6.0), 0.2 M calcium chloride dihydrate, and 20% (v/v) PEG 6000 |