6SEH

Recognition and processing of branched DNA substrates by Slx1-Slx4 nuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.2828
Spacegroup name	C 1 2 1
Unit cell lengths	180.322, 63.410, 103.687
Unit cell angles	90.00, 116.70, 90.00

Refinement procedure

Resolution	29.740 - 3.150
R-factor	0.2069
Rwork	0.202
R-free	0.25360
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.577
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.750	3.330
High resolution limit [Å]	3.150	3.150
Number of reflections	35689	5772
<I/σ(I)>	8.75	1.72
Completeness [%]	97.0	90
Redundancy	3.31	3.04
CC(1/2)	0.994	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298.15	0.1 M MES (pH 6.0), 0.2 M calcium chloride dihydrate, and 20% (v/v) PEG 6000