6SED

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.918400
Spacegroup name	P 31 2 1
Unit cell lengths	136.875, 136.875, 126.833
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.516 - 2.233
R-factor	0.1666
Rwork	0.165
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.002
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.516	46.516	2.220
High resolution limit [Å]	2.100	6.240	2.100
Rmerge	0.129	0.030	1.331
Rmeas	0.136	0.032	1.406
Total number of observations	808666
Number of reflections	80260	3233	12744
<I/σ(I)>	16.07	65.28	1.72
Completeness [%]	99.8	99.7	99.3
Redundancy	10.076	9.866	9.657
CC(1/2)	0.999	1.000	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	40% Tacsimate pH 6.0