6SEB
Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB in complex with IPTG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-27 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.918400 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 137.069, 137.069, 126.795 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.539 - 2.272 |
R-factor | 0.1588 |
Rwork | 0.157 |
R-free | 0.20540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6etz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.873 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.539 | 46.539 | 2.360 |
High resolution limit [Å] | 2.220 | 6.610 | 2.220 |
Rmerge | 0.122 | 0.027 | 1.277 |
Rmeas | 0.128 | 0.029 | 1.346 |
Total number of observations | 686545 | ||
Number of reflections | 67951 | 2765 | 10776 |
<I/σ(I)> | 17.26 | 68.48 | 1.74 |
Completeness [%] | 99.8 | 99.6 | 99 |
Redundancy | 10.104 | 9.542 | 10.057 |
CC(1/2) | 0.999 | 1.000 | 0.713 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40% Tacsimate pH 6.0 |