6SE7
R600A mutant from Mycoplasma genitalium P110 Adhesin at 1.87 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 109.393, 150.696, 176.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.360 - 1.870 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r3t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.520 | 2.080 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.039 | 0.730 |
| Number of reflections | 73762 | 3689 |
| <I/σ(I)> | 16.3 | 2 |
| Completeness [%] | 61.5 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350 0.2M Tri-potassium citrate |
