6SE5
Y830A mutant from Mycoplasma genitalium P110 adhesin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2019-02-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 109.322, 153.117, 169.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.300 - 2.210 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r3t |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.930 | 2.240 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.058 | 1.550 |
Number of reflections | 70593 | 3502 |
<I/σ(I)> | 12.5 | 0.6 |
Completeness [%] | 98.8 | 99 |
Redundancy | 4.6 | |
CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23 % PEG 3350 0.2 M Tri-potassium citrate |