6SD0

Structure of beta-galactosidase from Thermotoga maritima.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979470
Spacegroup name	I 4
Unit cell lengths	167.852, 167.852, 519.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.700
R-factor	0.2432
Rwork	0.241
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dp0
RMSD bond length	0.004
RMSD bond angle	1.444
Data reduction software	XDS
Data scaling software	Aimless (7.0.071)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.780
High resolution limit [Å]	3.700	3.700
Rmerge	0.165	1.740
Rmeas	0.182	1.906
Rpim	0.075	0.771
Number of reflections	76050	26943
<I/σ(I)>	10.1
Completeness [%]	99.8	99.9
Redundancy	5.8	6
CC(1/2)	0.996	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	12% PEG 3350, 0.1 M Bis-Tris propane pH 8.5, 0.2 M NaNO3, 2% MPD, 8 mM n-Nonyl-b-D-thiomaltoside. The crystals were enhanced by the microseeding technique.