6SCS

Cell Division Protein SepF in complex with C-terminal domain of FtsZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	34.246, 75.534, 52.614
Unit cell angles	90.00, 102.71, 90.00

Refinement procedure

Resolution	18.480 - 2.200
R-factor	0.208
Rwork	0.206
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.450	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.140	0.555
Number of reflections	13335	1155
<I/σ(I)>	8.8
Completeness [%]	99.8	100
Redundancy	6.7	6.6
CC(1/2)	0.996	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M MgCl2, 0.1M MES pH 6.5, 30% w/v PEG 400