6SCE
Structure of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.370, 84.520, 123.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.700 - 1.830 |
| R-factor | 0.17813 |
| Rwork | 0.176 |
| R-free | 0.20944 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.700 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.830 |
| Rmerge | 0.070 | 1.430 |
| Number of reflections | 78941 | 4460 |
| <I/σ(I)> | 18 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.4 |
| CC(1/2) | 0.999 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 3350, 0.2 M sodium citrate, 0.1 M bis-Tris propane, pH 6.5 |
